myoglobin levels

[J.Mols]

Does anybody know the differences of expression of muscular myoglobin between an apnea trained person and a non-trained one. I am also interested by the myoglobin levels of athletes trained to anaeroby like sprinters or to aeroby like long-distance runners, swimmers or cyclists.
I already know that a common person has around 5 mg of myoglobin per kg of fresh skelettal muscle. I have just been reading that seals have about 20 mg/kg and whales 70-100 mg/kg. Are there also great differences between different muscle groups and the type of muscles fibres (I, IIa, IIb, IIc and lately IIx)?

Thanks

J. Mols

[efattah]

Very good question. I have researched this extensively.

Human myoglobin levels, as you stated, vary between 4-7mg/g of dry muscle.

I heard that penguins, seals and whales are 60-81mg/g, but maybe you're right, and some go up to 100mg/g.

Myoglobin in elite human cyclists was found to be no different than myoglobin of couch potatoes.

Further, power training and endurance training were both found to actually reduce myoglobin levels in athletes.

The only method ever discovered to actually increase myoglobin levels was to do high intensity exercise in hypoxia. Even then, the two studies which showed that, didn't actually measure the before & after myoglobin levels, they simply proved that the mRNA for myoglobin increased after the high intensity exercise in hypoxia, meaning that the body was signalling to grow more myoglobin, assuming enough iron and other raw materials were available.

To my knowledge, no one has ever measured the myoglobin levels of freedivers. Muscle biopsies to measure myoglobin are extremely expensive.

Whoever can discover an efficient training exercise to increase myoglobin levels will slowly but surely develop an insurmountable advantage over any opponents, and he/she could reach unimagineable proficiency in recreational diving & spearfishing.

Remember that in penguins, who inhale before diving, they store more O2 in their myoglobin than in either their lungs or their blood.

Likewise, a human with myoglobin of 60mg/g dry muscle would essential have a 2nd breath of air stored in their body, essentially doubing their diving capacity.

The only way I can think to make progress in this area is to actually invest $1000-$3000 for two or three muscle biopsies on ourself, then try some high intensity apnea exercises, and then do another muscle biopsy a month or two later, and see if the exercise was actually working. However, if anyone goes through that and actually finds an exercise which works, I doubt he/she would be willing to share that info for free!

But we can hope I guess.

One thing is for certain; whenever someone does discover such a training exercise, freediving will be taken to a level unimagineable by us today. Three hundred meter dynamics, one hundred and forrty meter constant ballast dives, seventy meter spearfishing dives... etc...


Eric Fattah
BC, Canada

[Bindlestitch]

Eric and J.,
Could either of you recommend any good books on marine mammal physioligy? I know virtually nothing about the subject but I'm really interested in it.
Eric, I saw on a tv program recently that an emperor penguin can dive some 1500 ft and stay under for sixteen minutes. I now understand from your description of myoglobin why they can stay for so long, but how does their body cope with the pressure of a 1500 ft depth dive?
God bless,
Israel

[efattah]

The deepest dive on record for the Emperor Penguin is 565m (1854ft), a dive which lasted for 12 minutes. Shallower dives have been recorded for up to 22 minutes.

Emperor Penguins, although technically birds, cope with the pressure just like other diving mammals, with respect to the blood shunt, plasma shift, and so on. Further, it has been calculated that the Emperor Penguin's lungs contain a volume of air insufficient to hold enough nitrogen to cause decompression sickness.

The Emperor Penguin is the champion of all diving animals, in the sense that it can dive to a deeper depth per kilogram of body weight, by a large margin. For example, it weighs about 30kg, but it can dive to 565m+, whereas the next best small creature is a harbor seal (100-200kg), which can dive to 420m+. An elephant seal (1000kg) can dive to 1800m, still nowhere near the depth per unit body mass of the emperor penguin.

Futher, the torpedo shape of the penguin's body has been shown to have the lowest drag coefficient of any shape, man-made or nature-made.

Eric Fattah
BC, Canada

[Bindlestitch]

Good grief! 1800m that's 5904ft! That is amazing. It's just awe inspiring to learn about what God has created.
Thanks for the info Eric. I'm really getting interested in subjects such as this since joining this forum. You've been a great help.
Are there any books that deal just with the diving physiology of marine mamals that you know of?
Much obliged and God bless,
Israel

[ivan]

hi

I guess I have way more respect for marine animals now

cheers

[gerard]

Bindlestitch, just search in Google and you'll be surprised the amount of books displayed.

Good luck, gerard.

[J.Mols]

Sorry for the huge mistake in the units : g/kg or mg/g instead of mg/kg.
J. Mols

[Bindlestitch]

Originally posted by gerard
Bindlestitch, just search in Google and you'll be surprised the amount of books displayed.

Good luck, gerard.

Good suggestions Gerard. Will do.


J. Mol , I appologize if I hijacked your thread , it's my overzelous curiosity taking over again.

[gerard]

might be the key.

I read some research which states that:

"High intensity training in hypoxia further results in an increase of vascular endothelial growth factor (VEGF) mRNA, capillarity and myoglobin mRNA. These results suggest that hypoxia training results in improvements of the oxygen transfer capacity in skeletal muscle tissue. They thus offer a plausible explanation for the observation that effects of hypoxia training in athletes can best be demonstrated when performance tests are carried out in hypoxia. Beneficial effects of "training high - living low" for sea level performance of athletes can be inferred from the structural changes observed in muscle tissue; however, the functional improvements remain to be demonstrated directly"

(Hypoxia training for sea-level performance - Training high - living low
Hoppeler H, Vogt M
HYPOXIA: FROM GENES TO THE BEDSIDE
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
502: 61-73 2001)


The second article states that:

"Myoglobin, a protein with an important role in muscle oxidative metabolism, is increased in high altitude residents... We therefore tested the hypotheses that myoglobin allele frequencies differed in Tibetans, a long-resident human high-altitude population, compared with sea-level residents, and varied in relation to altitude among Tibetans. We obtained the sequence of exon 2 of the myoglobin gene in 146 Tibetans with greater than three generations of stable residence at altitude in rural Tibet. We compared the frequency of known polymorphic sites in this gene among Tibetans living at altitudes of 3000, 3700, and 4500 m and to allele frequencies previously obtained in 525 residents of Dallas, Texas. We also examined the association between different myoglobin genotypes and hemoglobin concentration, used as an index of myoglobin levels. The frequency of the myoglobin 79A allele was higher in the high altitude compared with the sea-level residents, but unchanged with increasing altitude among Tibetans..." (Analysis of the myoglobin gene in Tibetans living at high altitude
Moore LG, Zamudio S, Zhuang JG, Droma T, Shohet RV
HIGH ALTITUDE MEDICINE & BIOLOGY
3 (1): 39-47 SPR 2002)

It would be interesting training for a year in a freshwater lake (and of course right after training going to sea level again) in "Andes" and see any improvements.

Is anyone out there willing to sponsor a good looking and kind freediver?

Je, je.

Regards, gerard.

[Jersey Jim]

Hello all:
There has been some interesting reading here. A truly fascinating subject. I look forward to reading more from this forum on the matter. I would like to include my .02 on increasing myoglobin levels.
The beef industry supplements selenium and vitamin E to cattle in order to increase their myoglobin levels. I imagine it's for getting a more appealing dark red color to the meat. I always try to utilize vitamin rich foods over supplements due to better absorption. I use an incredibly potent form of selenium from Brazil nuts. They are said to be 250 times the content of many other foods. But I always use the "shelled" ones, as there are two regions in Brazil that grow the nuts. The exporters in the "selenium rich soil" region of central Brazil, export nuts still in the shell. The less potent region of western Brazil, export nuts out of the shell. I do not know if this is more than coincidence. I also take caution not to consume more than 1 or 2 nuts per day, as selenium can accumulate in the body to toxic levels. I keep them in the freezer and remove one or two for a few minutes before cracking.
Also, be sure to eat iron-rich foods for the myoglobin to bind to. Meat sources, opposed to non-heme sources, absorb better, but non-heme sources seem to absorb better when taken with meat sources. Also, take vitamin-C when eating iron-rich foods. This enhances absorption. Try to avoid coffee, tea or cola's when trying to absorb iron.
Since reading some good articles on this forum, I've begun hypoxic training on a Nordik Track ski machine. Someone was right when they said that form of training was very hard on the body. I've started out with just an hour of continuous workout, with the apnea portion lasting 30 seconds, and normal workout for 60 seconds. At the end I tried 30 sec. on and off apnea, and seemed to make no difference in the ability to keep up. One interesting part though, was just like in the water, apnea became easier after that magic 20 minute mark from starting. Maybe the spleen emptiess fully by then?
Please keep these great post coming, as Eric stated, if this myoglobin thing is mastered, the performance will soar to new heights. I look forward to more on this subject here.

Thanks,

Jim

[TMcKee]

The information gained from a muscle biopsy should be shared freely with any freediver, imho.

Like I stated in my burnout thread, I wait until i've done at least 20 minutes of run/walking before I do any apnea running. I don't know if this qualifies for extreme hypoxic training, but I do it. The full effects would probably take some time to feel though.

[J.Mols]

Recently, I have read in a biochemistry book specialised in physical activities that "intense hypoxia" can cause a rise in the skelettal muscles myoglobin mRNA of about 70%. The book was refering to two articles I have not yet the time to search for. The question is what intense hypoxia means for the authors.

[Adrian]

I asked a university professor specialized in food chemistry about any possible ways to increase myoglobin and he said that apparently selenium has a very narrow "safety" margin, in other words, only 10 times the normal dosage will result toxic for the body.

Also that selenium as far as he knew was only used for sintesizing "selenocisteina" (sorry I can't translate it into English) which is involved again in the sintesizing of a couple of enzimes used for protection against free radicals.

So it seems that at least in humans selenium doesn't have much of a relationship with myoglobin.

He also suggested that the best way to increase one's myoglobin, apart from training in altitude was to be a whale

Safety first.

Adrian

[Jersey Jim]

Adrian, thanks for the good input. Not exactly what I wanted to hear though.

Could anyone tell me if it is possible for myoglobin stored in unused slow-twitch muscles, to be recruited by the bloodstream and utilized elsewhere in the body during freediving? For example, can the myoglobin stored in the "arm tissues" help prolonge a dive session that is not requiring the use of the arms, or does that myoglobin only benefit the muscles where it is stored?

Thanks in advance,

Jim