Gerschman and Gilbert were the first to propose that oxygen toxicity is caused by the production of free radical intermediates in excessive concentrations during exposure to increased oxygen pressures. The initial involvement of these agents is now well established, and several excellent reviews have summarized the literature on the biochemistry of oxygen free radicals. Although exact mechanisms are not yet known, free radical intermediates including superoxide anions, hydrogen peroxide, hydroperoxy and hydroxyl radicals, and singlet oxygen are potentially toxic to cell membranes, enzymes, nucleic acids, and other cellular constituents. Along with better understanding of oxygen free radicals has come a greater awareness of the dependence of vital biologic processes on cellular antioxidant defenses such as superoxide dismutase, catalase, and the glutathione system. It is now thought that in the absence of these defenses, the same oxygen pressures required to sustain life would cause lethal oxygen poisoning.